The K-Mart near the Morgantown Mall in Monongalia County will close in Mid December. The store employs 100 people.
Parent company Sears Holdings says those employees will have the chance to apply for positions at other K-Mart and Sears stores.
It said it's trying to reduce costs through a new business model that focuses on integrated retail.
The K-Mart will start liquidating assets on Sunday, September 29.